Optima PRO De-salting Plate

The Optima PRO de-salting plate is designed for small-scale de-salting, buffer exchange and removal of low molecular weight materials (e.g. imidazole) from larger biomolecules, or macromolecules such as proteins. Optima PRO de-salting plates are gel filtration plates consisting of 800 μL bed volume in a standardized array for processing Protein samples >5kDa. Optima PRO de-salting plate can be used for the removal of DNA primers and fragments (up to 20 bases), nucleotides labelled with biotin, isotopes, and other assorted markers. 

The Optima PRO de-salting plate provides convenient small-scale preparation of protein samples post affinity purification, or prior to analysis techniques such as gel electrophoresis, liquid chromatography and LC-MS. Designed for imidazole removal, desalting and buffer exchange.

Product Name	Optima PRO De-salting Kit
Protein Application	Removal of salts, low molecular weight impurities (e.g. imidazole) and buffer exchange
DNA Application	Removal of DNA primers and fragments (up to 20 bases), buffers, nucleotides labelled with biotin, isotopes and other markers
Plate format	96 well
Mode of operation	Centrifugation (bench-top or floor model) Positive pressure e.g. Tecan Resolvex A200
Total processing time	Centrifugation – 8mins Tecan ResolveX A200 – 2mins
Optimal Centrifugation Conditions	900 x g
Gel Matrix	Zetadex G50 resin
Gel bed volume	800 μl
Removal of Salt: (200 μl 1M NaCl)	Up-to 99%
For processing proteins*	> 5 kDa
Protein Recovery	> 90%
Maximum sample volume	300 μl
Minimum sample volume	50 μl
Plastic	Polypropylene
Storage buffer	20 % Ethanol
Working pH	2 – 13
Storage conditions	4°C
Expiry	2 – 3 years
Catalogue Number	PRO96
Pack sizes	2, 10 and 50 de-salting plates

* Smaller proteins may need to be centrifuged longer in order to elute